HPC Module: primer3
Synopsis
Adds primer3 software to your environment.
| About This Software | |
|---|---|
| Official Site | http://primer3.sourceforge.net |
| Tags | Designing PCR Primers |
Installed Versions
| Version | Install Date | Default? |
|---|---|---|
| 2.3.7 | 2016-08-17 |
Description
Primer3 is a widely used program for designing PCR primers (PCR = "Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool in genetics and molecular biology. Primer3 can also design hybridization probes and sequencing primers.
PCR is used for many different goals. Consequently, primer3 has many different input parameters that you control and that tell primer3 exactly what characteristics make good primers for your goals.
Citation(s)
The authors of Primer3 request but do not require that use of this software be cited in publications as:
- Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M and Rozen SG. Primer3--new capabilities and interfaces. Nucleic Acids Res. 2012 Aug 1;40(15):e115. The paper is available at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424584/
and
- Koressaar T and Remm M. Enhancements and modifications of primer design program Primer3. Bioinformatics 2007;23(10):1289-1291. The paper is available at https://www.ncbi.nlm.nih.gov/pubmed/17379693
If you use masker function, please cite:
- Koressaar T, Lepamets M, Kaplinski L, Raime K, Andreson R and Remm M. Primer3_masker: integrating masking of template sequence with primer design software. Bioinformatics 2018;34(11):1937-1938. The paper is available at https://www.ncbi.nlm.nih.gov/pubmed/29360956
Source code available at https://github.com/primer3-org/primer3.
Category
| Library | Programming | Software | SysAdmin |
|---|---|---|---|